Was built in 1590-1592 year the mayor of the Jewish town, Mordechai Mazlom, who financed a broad restructuring ghetto style Renessans. The synagogue was built under the leadership of Josef shaft and Jude Goldsmid de Hertz. The original building was heavily damaged during the fire of 1689, after which the building was restored in the Baroque style. After it was rebuilt in neo-Gothic style by the project of A. Grotta in 1893-1905, the synagogue has lost much of Baroque traits. From the initial disposition in the Renaissance style preserved three-aisled layout of the main nave and added a women's gallery.

Currently the Maisel synagogue serves as the exhibition premises and storage of the Jewish Museum. The first part of the exhibition history of the Jews in Bohemia and Moravia from the settlement until the beginning of emancipation illustrates the history of the Jews in the Czech lands from the tenth century to the end of the eighteenth century. The introductory part introduces the historical information about the origin of the Jewish settlement in Bohemia and Moravia. As well as legal and social status of Jews in the medieval state. Special attention is given to the Renaissance, as associated with the construction of the synagogue, so named from their founder of Mordica Misla. Traditional Jewish enlightenment are the works of prominent scientists who had two rabbis and rectors of Talmudic schools in the Czech and Moravian Jewish communities (Rabbi Liva, David Oppenheim).