The Nativity of Theotokos monastery, or convent of the Nativity of the blessed virgin Mary in Grodno was built on the spot where once stood Prechistenskaya Church. Mention Prechistenskaya Church is found in 1506 in the "Acts of Western Russia." It was founded by Prince Glinski, passing from Kyiv voivode Dmytro Putyata funds for the maintenance of the temple and existed at the Church almshouses. About hospice also cared Sigismund II Augustus, commanded to pay for its needs the money from the income from the Royal estates. The Church also owned land in the olšany tract. In 1614, cornet Kuntsevich bequeathed to the Cathedral Church of our lady of the Big parade in Grodno.

In the XVII century the Orthodox Church was handed over to the Uniates. Moved here Vasilisa Sapieha with three for nuns founded in Grodno female Basilian monastery. In 1642, Metropolitan Anthony Seljava gave to the monastery the lands to the West Prechistenskaya Church.

Wooden Basilian Church burned down several times and rebuilt. Devastating fires occurred in 1647, 1654, 1720 and 1728 respectively.

After the transition Grodno under Russian jurisdiction, in 1843 Basilian became Orthodox monastery of the Nativity of Theotokos convent. To create it in Grodno moved from Orsha abbess of Athanasius with the nuns and novices. In 1860 the nuns organized, in the convent orphanage for girls.

In 1866, for the arrival of Emperor Alexander II in the monastery was built the Church of St. Sergius of Radonezh.

In 1870, in the convent there was most rare miracle of the myrrh-streaming list of the icon of our lady of Vladimir. The nuns realized that their monastery is blessed with a special grace. Myrrh that is exuded icon, was collected in a special container in the shape of a cross, which today is kept in the monastery. This icon in the First world war was evacuated to St. Petersburg. After the end of the First World war Grodno became a Polish city, but the monastery of the Nativity remained Orthodox. He was returned to the miracle-working Vladimir icon.

The monastery existed until 1960, when the nuns were evicted from their walls in Jirovecci the monastery, and the miracle-working Vladimir icon confiscated and taken to Russia. She was in the suburban temple in the village. Ermolino.

After the collapse of the USSR in 1992, the Nativity Church was reopened, the restoration of monastic churches, the monastery was returned to the wonderworking Vladimir icon. Started by peaceful monastic life, was opened Sunday school for children.