Florovsky monastery was first mentioned in documents of the sixteenth century, at least in 1566 was granted a Charter to Prince Constantine of Ostrog, according to which the territory of the monastery was transferred to the Archpriest Jacob Gulkevichi, which was reopened monastery (that is, it existed before). In 1682, already found a mention of the fact that the hem was the maiden monastery with two churches, one of which bore the name of the Martyr flora.

However, since the resumption of the activities of the monastery until the early eighteenth century, the Florovsky monastery was difficult with finances, so he practically did not develop. Only in 1712, after the closure of the women's resurrection monastery and translation of the nuns who lived there in the Florovsky monastery begins the monastery, as all private ownership of the monastery became the property of Florovsky.

Shortly after the transfer of the nuns in the Florovsky monastery begin to build a new one, now the stone Church of the ascension. After the consecration of the Church in 1732, the monastery began officially called the ascension Florovsky. In addition, the rest of the temple was made of wood, so burned in the famous Kiev fire in 1811. Next year from the Treasury funds were allocated for restoration of the monastery, on which were erected stone buildings. By the early twentieth century, the entire territory of the monastery was built of stone and wooden buildings (hospital, hospice and several other temples).

In the Soviet times the monastery was closed, damaging the Church of the Holy Trinity. The revival of the monastery began only during the German occupation, and although Kiev was liberated, the abode has never been closed, although it continued to suffer harassment from the authorities. Today it continues to evolve and develop the traditions of spirituality.