The Botanical garden of Pisa, spread over an area of 3 hectares, is a structural subdivision of the University of Pisa, open daily to the public. This is probably the oldest Botanical garden in the world.

The garden was established in 1544 under the initiative of the famous botanist Luca Ghini from Imola and with the financial support of the Grand Duke Cosimo I de ' Medici, when it was Europe's first University Botanical garden. In 1563 it was moved from the garden of the monastery of San Vito on the banks of the river (today this place is Arsenal Medici) closer to the monastery of Santa Marta in the North-Eastern part of the city, and in 1591 at the initiative of the head of Lorenzo Mazzega – to its present location in via Luca Ghini close to the famous Piazza del Duomo.

From the earliest days in the Botanical garden began to assemble a collection of natural objects (now the natural history Museum of Pisa), and opened the library, which is part of the University library. In addition, there is an excellent collection of portraits of Directors of the Botanical garden, collected over many centuries of its history, and one of the first greenhouses built in Italy from steel frames.

Modern Botanical garden of Pisa is divided into several sections, in which you can see flower beds, ponds, greenhouses, various buildings. Here are grown local and exotic species of plants both in greenhouses and outdoors. It also operates the arboretum. Attraction of the garden is a historic Botanical Institute, built in 1591-1595, whose rear facade is decorated with sea shells and ceramic mosaics in the style of the grotesque.